Is there anybody that has experience with p57 expression assay by flow cytometry?

02 February 2014 0 2K Report

In AML cell lines supposed to show induction of p57 expression after treatment but it showed decrease mean expression with slow proliferation. I used an indirect flow cytometry assay.

Badges
Science method

More Mazin Al-Asadi's questions See All

What are the possible ways / instruments that can be used to measure the light uniformity under the concentrator in the lab?

31 December 2018 7,938 0 View

Excuse me, How can we use flow meters to measure the concentrations of mixed gases?

I use the system in the attached file to mix different gases with air, but how can find the concentration of gas in the chamber.

11 December 2018 575 4 View

How to calculate kWh in a PV solar concentrator?

Hi All, I got confused in finding the proper method to calculate the kWh(Energy) that can my solar concentrator produce in a certain location. I 've got the Peak Sun Hours (PSH) calculated which...

08 September 2018 8,743 3 View

Compound Parabolic Concentrator design for solar collector?

I have designed a Compound Parabolic Concentration by using a 3D printer from Formlabs and the shape looks great. I wonder if anyone I wonder if anyone knows where can I get suitable aluminum...

31 December 2016 7,521 5 View

Could anybody help in interpreting an abnormal qPCR melting curve for CD34 primer?

I've got a melting curve showing tow peaks (attached file), a small one before the main one, which is seen in 3 cell lines very similarly while absent in the NTC (no template control). The problem...

06 July 2015 9,778 10 View

Transcriptume vs whole exome, which is more informative over the other?

Does whole exome sequencing add any more information over transcriptome sequencing? I am trying to compare expression profiling in AML cells before and after specific cytokine treatment.

31 December 2014 10,096 3 View

How can we reduce the background fluorescence in IF staining of AML cell line cells in suspension?

I am testing translocation of protein from the cytoplasm to the nucleus, I've got a lot of background nuclear fluorescence despite that I used 0.3M glycine in the blocking buffer.

05 June 2014 3,375 7 View

The inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria?

I studied for some time the inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria but did not get any result. Can a colleague help you with some advice?

01 January 1970 4,030 6 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View