Mazin Al-Asadi

4 Questions 8 Answers 0 Followers

Questions related from Mazin Al-Asadi

Could anybody help in interpreting an abnormal qPCR melting curve for CD34 primer?

I've got a melting curve showing tow peaks (attached file), a small one before the main one, which is seen in 3 cell lines very similarly while absent in the NTC (no template control). The problem...

07 July 2015 9,723 10 View

Transcriptume vs whole exome, which is more informative over the other?

Does whole exome sequencing add any more information over transcriptome sequencing? I am trying to compare expression profiling in AML cells before and after specific cytokine treatment.

01 January 2015 10,043 3 View

How can we reduce the background fluorescence in IF staining of AML cell line cells in suspension?

I am testing translocation of protein from the cytoplasm to the nucleus, I've got a lot of background nuclear fluorescence despite that I used 0.3M glycine in the blocking buffer.

06 June 2014 3,324 7 View

Is there anybody that has experience with p57 expression assay by flow cytometry?

In AML cell lines supposed to show induction of p57 expression after treatment but it showed decrease mean expression with slow proliferation. I used an indirect flow cytometry assay.

02 February 2014 1,447 0 View