Transcript is more for GUS gene, whereas GUS protein level is low in Real-time data. Do you have any explanation?

04 April 2015 1 6K Report

In my real-time experiments, transcripts for GUS assay is more, whereas the GUS protein level is less. The quantification was done by fluorometry. What can be the reason?

Prashanth N Suravajhala

Dear Brijesh

Whatsoever be it, generally you seldom find significant correlation between transcript and protein/phenotype data. That said RT PCR is always false positive driven. It all depends on how good the sampled data is

Regards

prash

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Science method

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