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I want to construct a phylogenetic tree using almost 2000 sequences from prokaryotes as well as eukaryotes. To simply the tree, I would like to shortlist the sequences. What is the best way...
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What are the methods to check chemical modifications in DNA or RNA? By chemical modifications I mean formation of some other compounds on reaction with other metabolites, or some external...
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We mix the two solutions in appropriate ratios to get the desired pH buffer. Why can't we adjust the pH using NaOH or HCl?
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In my real-time experiments, transcripts for GUS assay is more, whereas the GUS protein level is less. The quantification was done by fluorometry. What can be the reason?
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I have tried isolation, but have always faced difficulty while crushing. The manual says that we should crush the leaves in liq. N2, let liq. N2 evaporate and then immediately add AP1 (lysis)...
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I am trying to amplify AT-rich promoter with Taq polymerase. Have tried various approaches like varying MgCl2 concentration, changing annealing temperature, changed genomic DNA and primer...
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