MEGA software gives different options for tree construction. Can someone please explain when to use which tree? I read literature but couldn't understand clearly. Please suggest some literature where I can find the differences.
I want to construct a phylogenetic tree using almost 2000 sequences from prokaryotes as well as eukaryotes. To simply the tree, I would like to shortlist the sequences. What is the best way...
06 July 2017 3,927 5 View
What are the methods to check chemical modifications in DNA or RNA? By chemical modifications I mean formation of some other compounds on reaction with other metabolites, or some external...
04 May 2016 4,904 4 View
Can anyone tell me downstream genes for calcium and MAPK signaling cascade in plants that can be used as positive controls for qPCR?
09 October 2015 1,539 1 View
We mix the two solutions in appropriate ratios to get the desired pH buffer. Why can't we adjust the pH using NaOH or HCl?
08 September 2015 7,941 1 View
I have tried isolation, but have always faced difficulty while crushing. The manual says that we should crush the leaves in liq. N2, let liq. N2 evaporate and then immediately add AP1 (lysis)...
03 April 2015 5,939 14 View
In my real-time experiments, transcripts for GUS assay is more, whereas the GUS protein level is less. The quantification was done by fluorometry. What can be the reason?
03 April 2015 6,376 1 View
I am trying to amplify AT-rich promoter with Taq polymerase. Have tried various approaches like varying MgCl2 concentration, changing annealing temperature, changed genomic DNA and primer...
02 March 2015 6,544 7 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Is it necessary to include a literature review even though I can't find any research that intersects with my own?
31 July 2024 6,774 6 View
Dear colleagues, I wonder if combining different research methods, namely literature review, semi-structured interviews and structured questionnaire survey will help to ensure validity and...
30 July 2024 5,154 17 View
Context: I am writing a Systematic Literature Review (SLR) as part of my master's thesis project. After initially screening the titles and abstracts of 338 papers from four selected databases, I...
29 July 2024 9,370 4 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?
27 July 2024 5,455 2 View
Hello, I want to know how to get the templates from the Elsevier official website (their last updated if exists)? I would like to know the steps if possible.
26 July 2024 3,264 2 View
I am currently working on a regression model for a project and considering using both Random Forest and Decision Tree algorithms. Given that Random Forest is essentially an ensemble of Decision...
23 July 2024 4,306 3 View