To validate the differentiation of THP-1 to M0 & M1 macrophages?

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How effective are the Blue Carbon policies of the UK in contributing to sustainable development, and what are the potential trade-offs and synergies?

The aim of this research is to evaluate the effectiveness of Blue Carbon policies in the UK and their contribution to sustainable development, while identifying potential trade-offs and synergies...

16 March 2023 6,502 2 View

Can someone please recommend a sonicator to use in Chip-Seq experiment for chromatin shearing?

I am looking for a sonicator with a reasonable price for our lab, which I can get consistent results in DNA chromatin shearing. I appreciate any suggestions and your experiences with chromatin DNA...

24 May 2021 9,325 6 View

How do I get the whole p value in a meta-analyis using STATA?

In STATA version 16, when an association statistically significant results shows p value as 0.000 how do I get the exact value? I have used r(p_z) or return list. But not working.

09 March 2021 7,393 4 View

Has anybody used or know a "closed culture vessel" system to grow microorganisms by supplying different pressure conditions?

microbiology culture

08 February 2019 2,843 7 View

Can anyone help with the standards, controls, and samples in qPCR?

I need to run a series of qPCRs and have to optimise my primers before I start. Its all the more confusing when so many different protocols mention so many different terms, and I am now in a...

27 February 2014 5,507 4 View

Bursting default assumptions in the language/literature classrooms

As we all know, several default assumptions exist in the world. These could be assumptions pertaining gender, races, religions etc. and are often misleading and bigoted. Can you suggest some...

01 January 1970 3,746 1 View

What kind of functionalizing agents should be used for binding lipid/proteins on the substrate

Dear All, May be it is a silly question. I need to functionalize a glass surface for binding some parasite specific lipids and proteins. What kind of functionalizing agents could be used for the...

01 January 1970 2,599 3 View

What , accroding to you, are the most important things to bear in mind when it comes to cross cultural communication?

In a globalized world where communities are in constant contact with each other in an unprecedented manner, it is important to know how meanings vary across cultures. A lack of this knowledge can...

01 January 1970 8,011 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View