What kind of functionalizing agents should be used for binding lipid/proteins on the substrate

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Barry Arkles

There are number of glass functionalizing agents, call site coping agents that can work. It depends on the specific protein or lipid. See the following on p27: https://www.gelest.com/themencode-pdf-viewer/?file=https://www.gelest.com/wp-content/uploads/Goods-PDF-brochures-couplingagents.pdf

and https://www.gelest.com/themencode-pdf-viewer/?file=https://www.gelest.com/wp-content/uploads/Whole_cell_and_cell_organelle_immobilization_on_siliceous_substrates.pdf

Thulya Chakkumpulakkal Puthan Veettil

@Barry Arkles Thank you so much for your suggestion. Really appreciated. I have a question to ask. Are glutetaldehyde/APTES only binds with lipids/proteins or with whole cell?

Sharat Chandra Barman

Dear Thulya Chakkumpulakkal Puthan Veettil

Protein/aptamer/antibody can be bind onto the -COOH, -NH2, -CHO, thiols group functionalized surface.

For further information you can follow this articles

1. Article A Polyallylamine Anchored Amine-Rich Laser-Ablated Graphene ...

2. Article Trimetallic Pd@Au@Pt nanocomposites platform on -COOH termin...

3. https://iopscience.iop.org/article/10.1149/2.0361912jes/meta

Thank you