Hello, you can construct your pressure by osmotic way. If you use deep water for pressure you can use osmotic solutions which will give you similar conditions. Also you can use unaerostat or modified atmosfer chamber connected with compressor for making pressure inside.
Hello, all traditional Microbial cells growing in a tube or flask of liquid medium are said to be in a closed system. Here no nutrients are added to the system and no metabolic waste products are removed. When nutrients are added to the system, the cells initially divide by binary fission and the cell number increase for a period of time. Once the nutrients are over in the medium the growth eventually stops and certain metabolic waste products are accumulated in the medium.
A culture of microorganisms produced by inoculating a closed vessel containing a single batch of medium is called a batch culture. Because no fresh medium is provided during incubation, nutrient concentration decreases and concentration of waste products increases. The growth of microorganisms reproducing by binary fission can be plotted on the logarithm of the number of viable cells versus incubation time.
For you may be useful attached info about closed culture vessel" system
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Thank you Karen A. Darbinyan for your valuable response.
I am wondering whether is it possible to regulate the pressure of a culture system to mimic hydrostatic pressure certain barophilic microorganisms experience in deep water.
Hello, you can construct your pressure by osmotic way. If you use deep water for pressure you can use osmotic solutions which will give you similar conditions. Also you can use unaerostat or modified atmosfer chamber connected with compressor for making pressure inside.
There are lots of papers published with various apparatuses that are used to grow and test bacteria under various pressures. In fact there is even a book on the topic: High-Pressure Microbiology Editors: Chris Michiels, Douglas H. Bartlett, Abram Aersten punished by ASM (American Society for Microbiology). The choice of how to regulate the pressure in your growth chamber is probably going to be dependent upon what pressure range you are working in, what sort of organisms you are studying etc.
Hello there, it is an interesting topic. Initially, you may try your microbial culture in a French Press chamber (small in size) , which are generally used to disrupt cell with pressure, to obtain cell free extract for enzymes. I guess, you can vary pressure to some extent, depending on the pressure range you' re interested for. One more point is it depends on your purpose of using a pressure culture system: is it to study the synthetic behaviour of the enzymes/microorganism under pressure, or is it to supply a nutrient gas such as H2 or others of the type. If you decide the initial culture conditions, then you construct you pressure culture chamber, as you need. Good luck.
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