I am looking for a sonicator with a reasonable price for our lab, which I can get consistent results in DNA chromatin shearing. I appreciate any suggestions and your experiences with chromatin DNA sonication for the Chip-seq experiment. Thanks in advance.
We use an old model from diagenode (bioruptor plus), which can hold 6x 1.5ml tubes or 12x 0.5ml tubes and it gets us consistent results so far. There's an updated model, i think it is called bioruptor pico. Depends upon your sample types, you can choose either of them.
I used bioruptor pico and Covaris. I prefer bioruptor because you can hold many samples at time, it is easier to standardize your protocol and the results are very consistents.
I always used the Covaris S220 Ultrasonicator. It offered alot of settings and control. Also for sonication conditions, you need to optimize per cell line and find a balance between sonication of chromatin versus destroying proteins. Always check with RT-qPCR and Western for sonication settings.
I've used a tip-sonicator (e.g. Branson sonifier), Bioruptor (Diagenode), two different Covaris instruments, and enzymatic shearing (kit based, likely just MNase). They've all worked well. It just depends on your application and how much chromatin you have. Enzymatic shearing is really easy but takes longer. The Bioruptor, for me, created a lot more heat and sample agitation (basically splashing the sample up on the sides of the tubes). Larger sample volumes helps reduce this problem. I never had that issue with the Covaris (different tech), but they are much more expensive than a Bioruptor. If you have a core facility with one, I'd use that over other machines.
Dear Rukshana Mohamad-Ramshan for ChIP-seq I strongly recommend our Diagenode Bioruptor Pico.
If you would like to have more information and specifications do not hesitate to contact me
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