What is the best (proper) method to thaw buffy coat ?

03 April 2017 9,189 0 View

Copy number variation (CNV)-QuantaSoft software?

I want to ask concerning on QuantaSoft software. For copy number variation (CNV) what the type i have to choose for FAM as well for VIC.

02 March 2017 9,938 0 View

TaqMan Copy Number Assay?

How long to thaw the component for TaqMan Copy Number Assay? What is the rpm,degree Celsius, duration to centrifuge the component for TaqMan Copy Number Assay?

02 March 2017 3,819 4 View

How to dilute restriction enzyme EcoRV.?

So that i can assemble directly in ddPCR SuperMix

01 February 2017 5,523 2 View

Extraction of DNA?

Is it a necessary buffy coat I aliquots for extraction of DNA is added with RNA later prior to storage at -80C?

01 February 2017 1,114 4 View

Droplet digital PCR (ddPCR) ?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...

01 February 2017 2,171 23 View

Droplet digital PCR (ddPCR), supermix,probe?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...

01 February 2017 5,594 2 View

Digestion of DNA (Restriction Enzyme-EcoRV) ?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...

01 February 2017 4,411 5 View

Digestion of DNA?

Is it necessary to use MULTI_CORE 10X Buffer fro digestion of DNA?

01 February 2017 9,776 1 View

DNA digestion, storage,duration?

31 December 2016 2,997 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View