Water should be added first or the digested DNA?What is the order ?
Please Check: https://www.qiagen.com/us/resources/molecular-biology-methods/dna/
I stored my buffy coat sample at -20C. So,what is the best n proper way to thaw the sample?vortex?centrifuge?duration?
03 April 2017 9,258 0 View
I want to ask concerning on QuantaSoft software. For copy number variation (CNV) what the type i have to choose for FAM as well for VIC.
02 March 2017 10,076 0 View
How long to thaw the component for TaqMan Copy Number Assay? What is the rpm,degree Celsius, duration to centrifuge the component for TaqMan Copy Number Assay?
02 March 2017 3,890 4 View
Is it a necessary buffy coat I aliquots for extraction of DNA is added with RNA later prior to storage at -80C?
01 February 2017 1,189 4 View
So that i can assemble directly in ddPCR SuperMix
01 February 2017 5,647 2 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...
01 February 2017 5,753 2 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...
01 February 2017 2,289 23 View
Is it necessary to use MULTI_CORE 10X Buffer fro digestion of DNA?
01 February 2017 9,852 1 View
01 February 2017 4,490 5 View
How long can I stored the digested DNA at -200C ? And Then if I dilute the DNA digest, can i store at -200C? How long i can stored it(duration)? OR Is it better not to dilute the DNA and stored...
31 December 2016 3,088 6 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View