What is the best (proper) method to thaw buffy coat ?

03 April 2017 9,189 0 View

Copy number variation (CNV)-QuantaSoft software?

I want to ask concerning on QuantaSoft software. For copy number variation (CNV) what the type i have to choose for FAM as well for VIC.

02 March 2017 9,938 0 View

TaqMan Copy Number Assay?

How long to thaw the component for TaqMan Copy Number Assay? What is the rpm,degree Celsius, duration to centrifuge the component for TaqMan Copy Number Assay?

02 March 2017 3,819 4 View

How to dilute restriction enzyme EcoRV.?

So that i can assemble directly in ddPCR SuperMix

01 February 2017 5,523 2 View

Extraction of DNA?

Is it a necessary buffy coat I aliquots for extraction of DNA is added with RNA later prior to storage at -80C?

01 February 2017 1,114 4 View

Droplet digital PCR (ddPCR) ?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...

01 February 2017 2,171 23 View

Droplet digital PCR (ddPCR), supermix,probe?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...

01 February 2017 5,594 2 View

Digestion of DNA (Restriction Enzyme-EcoRV) ?

How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...

01 February 2017 4,411 5 View

DNA digestion, storage,duration?

31 December 2016 2,997 6 View

To dilute the DNA digest?

Water should be added first or the digested DNA?What is the order ?

31 December 2016 6,962 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View