How many times i can freeze and thaw? -For the component as below
How long to thaw? -For the component as below
Once i thawed can i stored back at -20C?-For the component as below
How I should minimize freeze and thaw?-For the component as below
RE (EcoRv), Buffer D, BSA and MULTI-CORE 10x Buffer
The enzyme is supplied in 50% glycerol and while stored at -20C, it does not freeze. Keep it as chilled as you can while using the enzyme but strictly speaking, you are not freezing-thawing the enzyme.
The rest of the components can be subjected to as many freeze-thaw cycles as you wish or necessary for your work.
The enzyme is supplied in 50% glycerol and while stored at -20C, it does not freeze. Keep it as chilled as you can while using the enzyme but strictly speaking, you are not freezing-thawing the enzyme.
The rest of the components can be subjected to as many freeze-thaw cycles as you wish or necessary for your work.
Dear Siti,
I strongly support Andrey's suggestion, aliquot-making is a generally adopted method. You can prepare 4-5 aliquots in order to thaw a vial when you need it, instead of doing it with the entire stock solution. It is crucial to thaw on ice in order to give a minimal shock to the molecule.
Good luck with your work,
Davide
I stored my buffy coat sample at -20C. So,what is the best n proper way to thaw the sample?vortex?centrifuge?duration?
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