I stored my buffy coat sample at -20C. So,what is the best n proper way to thaw the sample?vortex?centrifuge?duration?
I want to ask concerning on QuantaSoft software. For copy number variation (CNV) what the type i have to choose for FAM as well for VIC.
02 March 2017 10,076 0 View
How long to thaw the component for TaqMan Copy Number Assay? What is the rpm,degree Celsius, duration to centrifuge the component for TaqMan Copy Number Assay?
02 March 2017 3,890 4 View
Is it a necessary buffy coat I aliquots for extraction of DNA is added with RNA later prior to storage at -80C?
01 February 2017 1,189 4 View
So that i can assemble directly in ddPCR SuperMix
01 February 2017 5,647 2 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as belowOnce i thawed can i stored back at -20C?-For the component as belowHow I should...
01 February 2017 5,753 2 View
How many times i can freeze and thaw? -For the component as below How long to thaw? -For the component as below Once i thawed can i stored back at -20C?-For the component as below How I should...
01 February 2017 2,289 23 View
Is it necessary to use MULTI_CORE 10X Buffer fro digestion of DNA?
01 February 2017 9,852 1 View
01 February 2017 4,490 5 View
How long can I stored the digested DNA at -200C ? And Then if I dilute the DNA digest, can i store at -200C? How long i can stored it(duration)? OR Is it better not to dilute the DNA and stored...
31 December 2016 3,088 6 View
Water should be added first or the digested DNA?What is the order ?
31 December 2016 7,037 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View