How do I convert the .wiff file obtained from ABSciex instruments to a generic .txt file (or a .msp file) to be used in databases like Massbank?

Not able to find a way to convert the file for having all the MS MS spectra in a text file.

01 February 2014 5,051 5 View

We are about to buy an RTPCR. Does anyone have any advice?

10 November 2013 2,700 7 View

Where to purchase a cellometer?

Our lab is planning to purchase a cell counter for routine cell culture application, and we would appreciate any suggestions where to buy it.

10 November 2013 5,560 3 View

How can I separate clumpy Caco-2 cells?

I am working with Caco-2 cells for the first time. It seems that they are very clumpy and do not separate well even after passing through the stripette many times. It makes it difficult for me to...

06 July 2013 5,594 11 View

What is the minimum no. of biological replicates for proteomics experiments?

I work with porcine samples, unfortunately we only have 5 animals/treatments. Is it enough for a proteomics experiment, do I have sufficient technical reps?

31 December 2012 8,129 8 View

DNAse treatment in RNA extraction - Kunitz?

I am trying to use an optional DNA treatment from Amresco company, check attached documents for product details. I am not quite familiar with DNAse units. So I am confused. The product I bought...

11 December 2012 2,591 4 View

Is there any special protocol for RNA extraction from adipose tissue?

I use a commercial kit for RNA extraction, however I am not sure if it is suitable for adipose tissue. However, due to a high content of triglycerides do I need to have an extra step or something...

11 December 2012 3,867 7 View

Making our own ELISA plates

How I can make my own ELISA plate. I do have certain protocols but they seem to be basic and do not have any ideas in case anything goes wrong. I need an ELISA for Swine adiponectin and I cannot...

10 November 2012 1,712 23 View

Purchasing a real time PCR instrument

Our lab is planning to get a PCR instrument and we are planning to demo some instruments. Do any one here have experience of recently purchasing an instrument please share their thoughts, will...

07 August 2012 4,191 15 View

Method for extracting anthocyanins/phenolics from plasma for UPLC/PDA analysis

I am trying to show anthocyanin and its metabolites in porcine plasma using UPLC/PDA detector using aglycone and phenolic acid standards. I want any method for extracting anthocyanins/phenolics...

03 April 2012 1,828 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View