Sridhar Radhakrishnan

11 Questions 22 Answers 0 Followers

Questions related from Sridhar Radhakrishnan

How do I convert the .wiff file obtained from ABSciex instruments to a generic .txt file (or a .msp file) to be used in databases like Massbank?

Not able to find a way to convert the file for having all the MS MS spectra in a text file.

02 February 2014 5,110 5 View

We are about to buy an RTPCR. Does anyone have any advice?

Our lab wants to get an thermal cycler for routine RT purposes, not looking for anything fancy. Any one has heard any reviews of the MultiGene™ OptiMax Thermal Cycler?

11 November 2013 2,733 7 View

Where to purchase a cellometer?

Our lab is planning to purchase a cell counter for routine cell culture application, and we would appreciate any suggestions where to buy it.

11 November 2013 5,588 3 View

How can I separate clumpy Caco-2 cells?

I am working with Caco-2 cells for the first time. It seems that they are very clumpy and do not separate well even after passing through the stripette many times. It makes it difficult for me to...

07 July 2013 5,622 11 View

What is the minimum no. of biological replicates for proteomics experiments?

I work with porcine samples, unfortunately we only have 5 animals/treatments. Is it enough for a proteomics experiment, do I have sufficient technical reps?

01 January 2013 8,166 8 View

Is there any special protocol for RNA extraction from adipose tissue?

I use a commercial kit for RNA extraction, however I am not sure if it is suitable for adipose tissue. However, due to a high content of triglycerides do I need to have an extra step or something...

12 December 2012 3,896 7 View

DNAse treatment in RNA extraction - Kunitz?

I am trying to use an optional DNA treatment from Amresco company, check attached documents for product details. I am not quite familiar with DNAse units. So I am confused. The product I bought...

12 December 2012 2,637 4 View

TNFa measurement in colon tissue using ELISA

I have an TNFa ELISA assay suitable for use in plasma, serum, cell culture supernatants etc. Can I use the same ELISA for measuring TNFa levels in the colon tissue by simply extracting protein...

11 November 2012 6,591 6 View

Making our own ELISA plates

How I can make my own ELISA plate. I do have certain protocols but they seem to be basic and do not have any ideas in case anything goes wrong. I need an ELISA for Swine adiponectin and I cannot...

11 November 2012 1,740 23 View

Purchasing a real time PCR instrument

Our lab is planning to get a PCR instrument and we are planning to demo some instruments. Do any one here have experience of recently purchasing an instrument please share their thoughts, will...

08 August 2012 4,221 15 View

Method for extracting anthocyanins/phenolics from plasma for UPLC/PDA analysis

I am trying to show anthocyanin and its metabolites in porcine plasma using UPLC/PDA detector using aglycone and phenolic acid standards. I want any method for extracting anthocyanins/phenolics...

04 April 2012 1,860 6 View