Hi,
has anyone any general thoughts on the effects of agar firmness on plant development using apical stems in firm versus soft agar? Since auxins produced in the meristems and can be transported down the stem , if a cut off stem is placed in the agar then is it possible that in a firmer agar that the endogenous auxins can get sequestered around the cut end where its sitting in the agar since they are less able to diffuse thru the medium. changing auxin/cytokinin ratios and or concentrations with those already added to the medium. I'm tissue culturing Nepenthes and other carnivorous plants. thanks steve
The quality and quantity of agar do influence the growth. However, it is not possible to pinpoint the exect reasons. Most of the workers attribute this to the availability of water. Agar is also known to contain some of the factor that restricts the water availability.
As for the change in the ratio of the cytokining/auxin is concerned, this could have nigligible effect as the concentration in the medium is generally many folds more that the endogenous concentrations.
Anil
thanks Anil, I wasn't sure how much was produced. sometimes in the multipcation phase I can have quite a few plantlets growing in one jars so wasn't sure if there was a enough from all of them to make a difference
Use gellan gum like Phytagel/Clergel now a days hydrogels are available so far I have not seen any papers where hydrogels are used in plant tissue culture..
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