Steve Sardina

6 Questions 12 Answers 0 Followers

Questions related from Steve Sardina

Does anyone have a culture of Monascus purpureus culture?

I only found it on the atta but too high for someone who has retired. instead of buying buying red yeast supplements and not know the % of Monacolin K, I'd like to grow it in liquid culture,...

15 August 2023 2,868 1 View

Does anyone have any photos of the dissection of lateral meristems?

before I start trying to dissect out the lateral meristems of my plants to use in tissue culture, i'd like to know what it looks like. All I see online are cross sectional micro-graphs of the...

24 February 2017 8,427 4 View

Any thoughts on the use of nadcc verses bleach for disinfecting seeds/explants?

Hi all..I have only 2 choices to use to disinfect my seeds/explants. Either bleach or NADCC. I have read the for NADCC longer periods with lower conc is better. Also read adding sugar to encourage...

02 February 2017 3,822 11 View

Using pgr's in 2 phase system?

: I need to test out various conc of PGRs effect of my explants. Was thinking it would be easier to add the pgr's in solution onto the top of the agar the explants are on. So for example if I...

30 January 2017 241 7 View

Nutrient versus non-nutrient agar for seed germination?

Hi, I'm disinfecting Nepenthes seeds for tissue culturing and been getti g a low germination rate. Not sure if its my protocol 420mg/l Nadcc, with sugar for 12 hours or I'm getting old seeds...or...

12 January 2017 7,764 5 View

Tissue culturing plants - general thoughts on the effects of agar firmness on plant development?

Hi, has anyone any general thoughts on the effects of agar firmness on plant development using apical stems in firm versus soft agar? Since auxins produced in the meristems and can be...

11 January 2017 4,005 4 View