Is there any selective media to grow filamentous fungus only (not with yeast)?

22 November 2020 5,370 7 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

Can anyone suggest me AI and WSN integrated Simulators and Emulators which are more suitable for real time applications?

For building the prototype of any of the real-time applications, the algorithms mostly are AI based AI-based. Hence, it would be better if the integrated tool is available to develop the...

07 June 2020 8,580 6 View

What is the pebbling number of a cycle graph with 4 vertices?

i need the number of pebbles for a cycle graph with 4 vertices

24 September 2019 4,188 1 View

Anyone know where the micro tensile testing machine available?

In Chennai where micro tensile testing machine available

19 August 2019 3,926 2 View

How do I etch nichrome thin film on polyimide(kapton) substrate? Can someone explain the procedure?

I'm currently working on fabricating etched heating element for a project work.

08 August 2018 1,746 3 View

Is there an association between non-vegetarianism and violence?

Is there any scientific study of this possible association? There have been informal discussions and surmises, but a serious study with appropriate controls to cancel out extraneous factors is...

29 May 2018 903 3 View

What could be the possible reasons for getting 20 unit less value of mass of pure compound which is well charecterised by NMR?

My compound is well charecterised by NMR but in case of ESI-mass spectrum it is coming 20 unit less then the expected mass. Molecule is not having any associatio with water. I could not find any...

12 September 2017 6,260 2 View

Different significance values on SPSS and PLS SEM. Please help.

On SPSS I am getting a result that shows no significant impact of the independent variable on the dependent. But when I am running the data on SmartPLS I am getting a significant impact. Please...

24 July 2017 5,505 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View