Is there any selective media to grow filamentous fungus only (not with yeast)?

22 November 2020 5,370 7 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

What is the pebbling number of a cycle graph with 4 vertices?

i need the number of pebbles for a cycle graph with 4 vertices

24 September 2019 4,188 1 View

Anyone know where the micro tensile testing machine available?

In Chennai where micro tensile testing machine available

19 August 2019 3,926 2 View

How do I etch nichrome thin film on polyimide(kapton) substrate? Can someone explain the procedure?

I'm currently working on fabricating etched heating element for a project work.

08 August 2018 1,746 3 View

Is there an association between non-vegetarianism and violence?

Is there any scientific study of this possible association? There have been informal discussions and surmises, but a serious study with appropriate controls to cancel out extraneous factors is...

29 May 2018 903 3 View

What could be the possible reasons for getting 20 unit less value of mass of pure compound which is well charecterised by NMR?

My compound is well charecterised by NMR but in case of ESI-mass spectrum it is coming 20 unit less then the expected mass. Molecule is not having any associatio with water. I could not find any...

12 September 2017 6,260 2 View

Different significance values on SPSS and PLS SEM. Please help.

On SPSS I am getting a result that shows no significant impact of the independent variable on the dependent. But when I am running the data on SmartPLS I am getting a significant impact. Please...

24 July 2017 5,505 3 View

Is gender a dichotomous variable? If yes, Can t-test be run on single large sample dichotomous variable (demographic)?

My colleague's thesis consists of a situation where she has performed a t-test to analyse the differences between male and female respondents' responses with respect to all the independent and...

19 July 2017 2,255 6 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

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22 February 2021 9,932 11 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View