The detailed Western Blot procedure

The membrane is first rinsed briefly with 20ml of TBS, followed by a 30-minute incubation, shaking with 10ml of Blocking Buffer. During this incubation, the volume of primary antibody needed for a 1:5000 dilution into 10ml of blocking buffer is calculated, and 10ml of blocking buffer is prepared for the antibody. Subsequently, the Blocking Buffer is removed, and the membrane is incubated for 45 minutes, shaking with 10ml of primary antibody in Blocking Buffer (Rabbit anti-ADH diluted 1:5000). After removing the antibody, the membrane is washed three times for 5 minutes each with 10ml of Blocking Buffer. Meanwhile, a 1:5000 dilution of the secondary antibody in 10ml of blocking buffer is prepared. The membrane is then incubated for 45 minutes, shaking with 10ml of secondary antibody in Blocking Buffer (Alkaline Phosphatase conjugated Goat anti Rabbit diluted 1:5000). Following the antibody removal, the membrane undergoes three 5-minute washes with 10ml of TBS/T. Finally, 5ml of BCIP/NBT liquid substrate (Sigma-B1911) is added, and incubation continues until color develops, with the reaction being stopped by rinsing with distilled water.

I was told I might have washed it with a different TBS (10mM one instead of 5mM)

What could the reason for such a different bands on the well.