Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How can I remove SDS from protein samples to be analyzed by reversed-phase LC?

Hi all, I want to characterize a product-related impurity band appearing in SDS-PAGE analysis. I excised the band and incubated it in elution buffer containing 0.1% SDS. Now I want to analyze the...

04 December 2020 1,798 3 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

How do you keep your incubator water bath free of organisms?

Hello, I keep my mammalian cells in a typical incubator at 37C with 5% CO2. I fill the water bath with diH20. Following the advise of a colleague, I add a few drops (250-500uL) of 20% SDS to...

02 October 2020 9,332 3 View

Can anyone suggest a protocol for extraction of nuclear proteins from cancer cell line without kits and detergent solution??

Can anyone suggest a protocol for extraction of nuclear and cytoplasmic proteins from cell line(H295R) without kits and detergent solution?

04 September 2020 1,364 2 View

What is the proper solvent which used in liquid detergent to make it soluble?

Good afternoon everyone, I hope you are doing well I had several samples of liquid detergent I wanna to soluble it with a solvent. I tried with both of ( water, Ethyl alc., Parrafin Oil, DPG,...

05 August 2020 8,664 3 View

Osmotic or detergent lysis for Intra-cellular vesicles Purification?

Hi, All the publications using vesicles purification, start with an osmotic lysis. For exemple this protocol : https://www.nature.com/articles/nprot.2013.053 Here, they use the following...

14 July 2020 5,047 3 View

Detergent-free lysate + loading buffer: adequate for western blots of membrane proteins?

I want to extract membrane+cytosolic proteins for western blots. I'm working with hardy bacteria, so I have to bead beat for full lysis. During bead beating, the manufacturer protocol recommends...

03 March 2020 4,743 20 View

Tinocat TRS KB2 or MnTACN ,which is better for powder laundry detergents?

In the powder detergents research we use system TAED/percarbonate. Idea is Catalyst/percarbonate. Tinocat TRS KB2 from BASF is not in product range just Tinocat LT200/or B249. MnTACN is available .

10 November 2019 4,268 2 View

Which detergent interferes with enzymatic activity the least?

I am doing an assay for interaction with a substrate and I need a detergent that will interfere with the enzymatic activity as little as possible. What is the general recommendation in this case....

10 November 2019 9,907 6 View