I am looking at cell type specific glucose absorption via immunofluorescence staining. When looking at my samples before I start staining, the fluorescently labeled glucose is within the cytoplasm and the signal looks bright and clear. However, once I go through the staining process, the signal is faded and diffuse around the nuclei. I can still estimate which cell the glucose came from but if there is a way to perform nuclear stains while keeping the cell membrane intact, that would be ideal. Any information regarding this is greatly appreciated, thank you!
Hello,
Regular nuclear stains such as DAPI or Hoescht are cell permeable, meaning that you don't need to permeabilize the plasma membrane when you want to use them. Be careful to dilute them well (1/10000 or so) to avoid a too strong fluorescence signal.
Hope this helps,
Nicolas
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