Is there any phosphates sensor (or total phosphorus) for online monitoring of the lakes?

We need to monitor surface waters in terms of nutrients and algae content. We are building up an on-line in-situ monitoring system for early detection of cyanobacterial blooming and toxicity into...

27 February 2020 9,612 7 View

Would you be interested in hosting a Fulbright Visiting Scholar?

I would like to apply for a Fulbright Visiting Scholarship, but I need a colleague who would like to host me in a U.S. University. My research interests include Special and Inclusive Education,...

08 October 2019 3,089 3 View

Best method for dna extraction from honey ?

hello, what is the best method for extracting bacterial dna from honey? thank youu

19 February 2019 131 6 View

Does anyone have any experience with RET immunohistochemistry?

Does anyone have any experience with RET immunohistochemistry? I am interested in staining nerves in skin. Thank you

09 November 2016 7,648 2 View

Is there a way to increase the sensitivity in the "do it yourself/ready-set-go" kits?

19 October 2016 8,696 3 View

What demethylating reagent do you suggest?

I would like to demethylate a substrate which carries one aryl methoxy group and heterocycle moieties. I tried some methods by using HBr and BBr3 but they didn't give any demethylated product. My...

03 August 2016 4,711 19 View

Is anybody working on architectural spaces and/or urban places or neighbourhood projects aiming at well-being of people with dementia condition?

02 August 2016 7,382 8 View

What is the best survey/questionnaire to assess children's perceptions on weapons?

We are conducting a study on primary school children's perceptions of what constitutes a weapon in Greece.

02 July 2015 1,407 2 View

Can I use boron tribromide for the demethylation of a methoxy group on a compound which carries also a primary arylamine?

Do I have to protect the amine?

28 July 2014 3,785 3 View

Imaging extracellular membrane proteins

I want to visualize a protein which will reach the plasma membrane and localize on the extracellular space while performing live cell imaging. Is there a way to have a tag, which will only become...

03 November 2013 582 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View