I want to prepare dilutions of serum samples and store them for months if necessary. However, I am concerned if serum proteins bind the walls of the eppendorf tubes or glass vials and effectively result in a drop in the original protein concentration. Can anyone suggest a container type that will not interact or bind with serum proteins? Is glass better then plastic tubes?
Many companies sell low protein binding tubes. For instance:
https://www.thermofisher.com/order/catalog/product/90410
Ideally, silane treated polypropylene or glass tubes will be fine. You can either treat tubes yourself or buy them pre-treated.
If incorporation of a carrier protein, such as BSA at 1mg/ml or more, is possible in your buffer for dilution, that will simplify things a great for you. You should be able to use any polypropylene tubes.
Dear Osman Duloca,
I suggest that you use glass tubes.
Best regards,
Kazemi
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