I'm trying to standarize TCE free staining method to visualize protein bands over SDS-PAGE, it's never been used in our lab. I've seen many published papers using this method, and the optimal TCE % (v/v) reported seems to be around 0.5% TCE. However, TCE stock solution molarity is not mentioned, not that I've seen. The amount of TCE I got is ammount limiting, so errores are not allowed.

Would any of you, colleagues that has used this staining method, be so kind and help me out giving me a glimpse about which is the most common TCE stock solution molarity used with SDS-PAGE?

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