These biofertilizers consist of a combination of microorganisms blended in either a powder or liquid form. The accuracy of conventional methods, such as plate counting and most probable number, can be inconsistent when dealing with multiple strains. This can make the task of quantifying microorganisms even more challenging.st appropriate method for different types of biofertilizers.
Quantifying microorganisms in dry substrate biofertilizers can be challenging due to the heterogeneous nature of the substrate and the presence of various components. However, there are several effective approaches for quantifying microorganisms in such biofertilizers. The method depends on the specific microorganisms of interest and the available resources. Some commonly used approaches are Plate Counting (CFU Method), Most Probable Number (MPN) Method, Quantitative PCR (qPCR), Fluorescence Microscopy and Plate Counting with Specific Media, etc.
Traditional methods for quantification of microorganisms rely on counting serially diluted plates assays (bacteria), direct counting by optical microscopy chambers (bacteria and protozoa), transmission electron microscopy (viruses), plaque assays (viruses and bacteria), endpoint dilution and hem agglutination assays. viable, plate count method and spectrophotometric (turbid metric) analysis. The number of visible colonies (CFU) present on an agar plate can be multiplied by the dilution factor to provide the CFU/ml value. Methods for counting the bacteria, measuring wet or dry mass, and measuring turbidity. equipment and supplies to conduct at least one of these experiments
1. Observing bacteria directly
2. Measuring wet OR dry mass
3. Measuring Turbidity
Foolowing link is usefull for quantifying bacteria
https://www.wikihow.com/Measure-Bacterial-Growth
The colony counts are made by doing dilution plating combined with analysis of the types of microbes in your preparation.
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