I want to induce a conditional knockout in pancreatic beta cells. I fed my mice on Tamoxifen containing diet starting from 4 weeks of age for 1 month as this tamoxifen administration protocol is established in my lab and works well with other cre/lox in other tissues but it didn't work for my system.I am thinking of giving tamoxifen as intraperitoneal injection following the dose mentioned in reference article that describes the cre mouse model I use. Do you think it might work better?
Hello!!
Check this out:
https://www.jax.org/research-and-faculty/tools/cre-repository/tamoxifen
This should work. I have used it with my cre/lox mice.
Good luck!!
Injections (every 24h for 5 days) should work better. there is a very good publication about cre/lox mice methods available here at ResearchGate. They used TAM injections
https://www.researchgate.net/publication/24182099_Inducible_Cre_Mice
We used 4OH-TAm.
Article Inducible Cre Mice
I have seen 5 days injection in majority of papers. I believe most of people follow injection method.
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