Dina Mostafa

6 Questions 11 Answers 0 Followers

Questions related from Dina Mostafa

Is it OK that Ins1 and Ins2 cycle threshold in qPCR are less than GAPDH in beta cells?

I am using ddCT method in qPCR for comparative analysis of Ins1 and Ins2 in beta cells of two groups of mice. I m using GAPDH as internal control. I wonder if it is OK and expected that Ins1 and...

28 November 2017 8,817 1 View

Tamoxifen inducible cre/lox?

I use tamoxifen inducible cre/lox system to induce knock out in vivo of my protein of interest. I feed my mice on tamoxifen containing chow for 1 month then I evaluate the KO. I do insulin and...

23 August 2017 4,456 2 View

Has anybody faced difficulties with islets isolation from db/db mouse model?

Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...

07 February 2017 1,809 7 View

What is the best internal control for western blots of mice lysates?

I have been using tubulin as internal control for my western blotting analysis of mice islet lysates. I wonder if there is a better internal control the researchers working with mice islets use.

29 October 2016 6,407 3 View

Does fasting affect the size of islets isolated from mice?

I recently started islets isolation and I am interested in investigating the effect of fasting on my target protein. I just noticed the islets I isolate from fasted mice are smaller than those...

28 October 2016 4,769 2 View

I am asking if somebody previously transfected islets of Langerhans isolated from mice with siRNA before. Is this going to work?

If this works what kind of tranfection reagent is recommended for the islets?

10 August 2016 466 8 View