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Questions related from Dina Mostafa
I am using ddCT method in qPCR for comparative analysis of Ins1 and Ins2 in beta cells of two groups of mice. I m using GAPDH as internal control. I wonder if it is OK and expected that Ins1 and...
28 November 2017 8,865 1 View
I use tamoxifen inducible cre/lox system to induce knock out in vivo of my protein of interest. I feed my mice on tamoxifen containing chow for 1 month then I evaluate the KO. I do insulin and...
23 August 2017 4,494 2 View
Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...
07 February 2017 1,842 7 View
I want to induce a conditional knockout in pancreatic beta cells. I fed my mice on Tamoxifen containing diet starting from 4 weeks of age for 1 month as this tamoxifen administration protocol is...
13 December 2016 4,599 3 View
I have been using tubulin as internal control for my western blotting analysis of mice islet lysates. I wonder if there is a better internal control the researchers working with mice islets use.
29 October 2016 6,446 3 View
I recently started islets isolation and I am interested in investigating the effect of fasting on my target protein. I just noticed the islets I isolate from fasted mice are smaller than those...
28 October 2016 4,838 2 View
If this works what kind of tranfection reagent is recommended for the islets?
10 August 2016 494 8 View
