Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Do we need to keep looking for the fracture angle after the Puck's failure initiation is reached?

Greetings to everyone, Our team is currently working on the development of a VUMAT code in Abaqus for composite modelling using the Puck's failure theory as damage initiation criteria. Currently...

20 January 2021 6,367 1 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Is there any specific software available for graphical presentation of signaling pathways/cycle in cells?

I'm planning to write a Review article, to make it more worthy and easy to understand for the targeted audience, I'm thinking to add some signalling pathways, and unfortunately, I have no idea how...

30 December 2020 6,395 2 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View