Jan Piwowarski

6 Questions 258 Answers 0 Followers

Questions related from Jan Piwowarski

Any tricks about enveloped VLPs protein solubilisation and purification?

Human coronavirus VLPs is composed out of 3 subunits with transmembrane domains, most likely co-purifies with membranes. The particle co-expressed in baculovirus is not soluble when purified in...

06 June 2015 8,102 5 View

Trouble-shooting guide about BS3 (Bis[sulfosuccinimidyl] suberate) protein cross-link. Any tips/tricks for beginners?

My group have nicely established glutardehyde cross-link to study the complex formation of the protein of our interest.We are working on both total tissue/cell extracts or recombinant proteins,...

10 October 2014 2,641 4 View

Western-Blot on p53 in tumor tissue - could you please provide references?

After two days of literature searching I’m not able to find any articles with p53 visualized in tumor/cancer tissue by Western-Blot. All I have found are showing p53 in cell lines only. Please...

10 October 2013 10,000 4 View

Does it make sense to reuse buffers/chemicals in Molecular Biology?

What is more efficient, to spare some chemicals and time for buffer preparation, or to make sure that the solution is always fresh? at which point is the buffer already too old? Any interesting...

09 September 2013 9,432 22 View

What is the concentration of TCEP/DTT/BME you add to SDS-PAGE loading buffer?

Any clue how resistant is it (boiling, long storage of the sample collection in the Laemli buffer)? This question I'm asking before the preparation of huge number of mammalian tissues collection...

08 August 2013 2,470 9 View

Can you show the picture of your worst Western blot, and explain the reasons for this disaster please?

I start this topic in order to collect the gallery of the photos of Western-blots (WB) as ugly as possible. The detailed description of the problem and the technical solutions were applied to...

08 August 2013 4,522 62 View