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Human coronavirus VLPs is composed out of 3 subunits with transmembrane domains, most likely co-purifies with membranes. The particle co-expressed in baculovirus is not soluble when purified in...
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My group have nicely established glutardehyde cross-link to study the complex formation of the protein of our interest.We are working on both total tissue/cell extracts or recombinant proteins,...
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After two days of literature searching I’m not able to find any articles with p53 visualized in tumor/cancer tissue by Western-Blot. All I have found are showing p53 in cell lines only. Please...
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What is more efficient, to spare some chemicals and time for buffer preparation, or to make sure that the solution is always fresh? at which point is the buffer already too old? Any interesting...
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Any clue how resistant is it (boiling, long storage of the sample collection in the Laemli buffer)? This question I'm asking before the preparation of huge number of mammalian tissues collection...
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I start this topic in order to collect the gallery of the photos of Western-blots (WB) as ugly as possible. The detailed description of the problem and the technical solutions were applied to...
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