Dear all,
I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks in advance!
Hello!
Please You look at this references:
Choice of Normal Ovarian Control Influences Determination of Differentially Expressed Genes in Ovarian Cancer Expression Profiling Studies
https://www.atcc.org/~/media/PDFs/Cancer%20and%20Normal%20cell%20lines%20tables/Gynecological%20cancer%20and%20normal%20cell%20lines.ashx
Hi Le Cong Truc,
When selecting a cell line is important to make sure that it is a good model for the disease you are studding. Normally we do a literature check to see what cell lines are being used for ovary carcinogenesis research.
It is also important to check the specific genetic background of the cell lines to know the organ of origin, differentiation type, mutations, growth rates, and other properties that will make a difference when planning the experiments and interpreting your results. For instance, some cell lines are not ideal to be used in a classical 96 well plate/72h proliferation assay because they don´t grow in low density.
You can check all the cell lines commercially available and its features in ATTC and Cellosaurus.
ATTC (https://www.lgcstandards-atcc.org/Products/Cells_and_Microorganisms/Cell_Lines.aspx?geo_country=es)
Cellosaurus (https://web.expasy.org/cellosaurus/)
Good luck!
Priscila
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line