To be developed.
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...
04 January 2021 3,591 3 View
I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan
01 January 2021 5,798 3 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...
01 December 2020 1,178 3 View
Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...
08 November 2020 9,100 3 View
Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.
01 November 2020 8,869 2 View
22 October 2020 9,354 4 View
I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...
10 October 2020 1,830 3 View
In GC-MS/MS I run the pesticide mix standard (in Acetonitrile) using SIM mode in which sharp well seperated peaks appeared, but there are ups and downs in base line. The same problem comes when...
08 September 2020 3,935 6 View
Examples of how to increase the contact surface area of the bottom area? Thank you.
25 March 2020 9,187 4 View
I developed a BFC (GOD anode and BOD cathode), tested it with apple and mouse's blood, the OCP with apple was 0.62V, but the OCP with mouse's blood was only 0.5V, I thought the OCP should be same...
25 March 2020 2,866 1 View
Conferences about agriculture, horticulture, tissue culture of apple.
20 February 2020 8,214 8 View
09 October 2019 3,400 5 View
This is probably going to seem silly, but I'm wondering which method of ANOVA to use in SPSS. I've carried out an experiment that contains two treatments (control vs. treatment). Subjects are...
08 September 2019 2,906 3 View
To be a good researcher or an expert, you should be curious , like Newton, when He noticed that an Apple fell on the ground, he asked Why did it happen? what are the causes of this fall ? What is...
03 April 2019 988 2 View
I need to know the pH of apple skin (not the flesh) and I cannot seem to find it anywhere. If you know it or can direct me to where I can learn more that would be appreciated. Thanks!
30 March 2019 3,559 3 View
02 March 2019 5,990 3 View
Sometimes when I eating an apple, I feel that the the lower part (blossom) of the apple more sweeter, whereas the upper part (middle to top) is often more watery and feels less sweet than lower...
19 February 2019 711 3 View