Hi all, I would like to ask if anyone has protocol for SDS PAGE sample preparation which the protein resuspend in organic solvent?
Dear Bee Yi Tan,
THESE ARE TWO ARTICLE FIND USEFUL FOR YOUR QUERY
1. https://www.nationaldiagnostics.com/electrophoresis/article/sample-preparation-sds-page
2. http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6194.pdf
Hope this willsolve your query
Many literatures have shown that the RVE model shows the grain boundaries between different grains. How can this be achieved using DAMASK+paraview?
21 July 2024 9,224 1 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Been doing searches to find the best paper to start understanding the advantages and disadvantages of each technology: Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with...
29 June 2024 9,856 1 View
Hi , in my study I will compare the effectiveness of Al generated feedback and traditional feedback in writing lesson.It will be a quasi-experimental design,but l have 3 classes not two.What...
25 June 2024 6,244 3 View
If we find sulfides in subduction zone interface rocks, how can we distinguish whether these sulfides are related to crust-derived fluids or originate from the original mantle wedge? Especially...
15 June 2024 654 1 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...
15 May 2024 1,458 3 View
Hi Research Gate people! I am trying to determine the sample size, power and alpha boundary needed for my interim analysis for a registered report in a psych journal. I have read the paper by...
28 April 2024 3,444 2 View
X=categorical IV (X1, X2) M1,M2,Y=continuous variables First: 1. compare X1 and X2, and hypothesize that X1 will predict higher Y 2. X1 will predict M1 3. M1 will predict M2 4. X1 will predict...
26 April 2024 6,207 0 View
As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...
23 April 2024 6,351 3 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View