Adam M. Passman

6 Questions 21 Answers 0 Followers

Questions related from Adam M. Passman

Has anyone used a non-biliary hepatocyte marker for IHC that works on human frozen tissue?

Can anyone recommend an hepatocyte antibody for IHC that they have used on frozen human liver? There are markers that will stain hepatocytes and cholangiocytes, but I would like to stain only...

12 December 2019 7,695 2 View

Why am I getting inconsistencies in my PCR duplicates?

I am amplifying a ~9kb mitochondrial product and keep getting inconsistencies in technical duplicates. In the attached image, I have 6 samples (#1-6; 1ng template from laser-captured tissue) #7's...

02 February 2019 2,900 12 View

Why am I losing DNA using QIAamp DNA Micro kit?

I'm having an issue that is possibly due to the QIAamp DNA extraction? I am extracting DNA from laser capture microdissection (LCM) of frozen section material. What I have been doing is cutting...

02 February 2019 7,840 9 View

Can someone recommend a protocol for extracting RNA from stained laser microdissected samples?

I'm aiming to extract RNA from laser microdissected samples. Can anyone offer advice or point me to a protocol that works? I want to be able to dissect stained (IHC) cells from a section (I've...

08 August 2013 8,250 2 View

Can anyone recommend an HIF1 reporter that works in mice?

I'm culturing cells in a hypoxia chamber and would like to see if I am inducing HIF1 expression in my mouse cells. Has anyone used an HIF1 reporter in mice that they can recommend?

06 June 2013 6,846 2 View

Cell fractionation for Western Blot - protocol

I've been looking at protocols to do cell fractionation. I want to be able to do Western Blots on both the cytosolic and nuclear fractions. Currently I'm not seeing any of my protein in the...

02 February 2013 4,155 11 View