I have prepared an SDS lysis buffer containing the following ingredients:
sucrose 30%
SDS 2%
EDTA 50mM
Tris-HCl 0.1M
Should I store it at room temperature or 4 C?
We store any SDS containing solution only under room temperature, that’s SDS may come out in low temperature.
Room temperature
Hi
Like Mu-Shen Chang has mentioned that SDS containing solution gets precipitated at lower temperature and therefore must be stored at room temperature. BTW what are u trying to lyse that needs 2% SDS?
Vikrant Mehta Hi the buffer is used for fungal cell lysis
Thank you for the helpful information
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