Tushar Chakraborty

3 Questions 119 Answers 0 Followers

Questions related from Tushar Chakraborty

Decrease in fluorescence anisotropy upon increasing metal ion concentration ?

I want to determine the binding affinity of my protein-metal ion complex using anisotropy. The protein has been FITC-labelled. I am using 500nM protein concentration. The anisotropy value...

29 August 2021 9,493 1 View

Can an isolated protein domain give a single band in SDS-PAGE equivalent to its dimeric mol. wt. ?

The protein I'm working with forms a homodimer and is a 2-domain protein. I have cloned and purified one of its domains. However, when I run the domain on SDS-PAGE, I get a single band equivalent...

20 August 2020 8,135 14 View

Can I prepare/download the dimeric structure of a protein which has a PDB file of the monomeric structure?

I want the dimeric structure of a protein (forms dimer) in .pdb format. The crystal structure of the protein is solved. However, the .pdb file at RCSB PDB is of the monomeric structure. Thanks...

10 July 2020 3,307 4 View