Best qualitative method for sequential explanatory dissertation?

Hello, I am conducting a dissertation using a sequential explanatory design. The order of the design is quantitative, quantitative, qualitative with more or less equal emphasis on each phase,...

14 March 2020 8,153 3 View

Can I use touchdown PCR for a 16S microbiome sequencing study?

Hi everyone. Just looking for some thoughts on this. My thinking is that I shouldn't use touchdown PCR to amplify 16S regions in this case because it will cause bias. The primers will already...

09 January 2020 8,577 1 View

Is salt to generate chlorine for swimming pools toxic?

I am trying to aquire recent research, that either confirms or disproves, that salt used to generate chlorine for swimming pools is more toxic to humans than standard clorine. Thanks! David

27 May 2019 8,034 3 View

Journal suggestions needed for parasite prevalence in Sargassum-associated shrimp?

Long story short, my MS thesis looked at Sargassum associated macrofauna. My fellow collaborators and I are currently writing everything up for publication as part of larger study but during the...

28 August 2018 793 1 View

How to get individual pixel values from line ROI's in ENVI?

I have 23 lines in my ROI, I want to extract the values for each individual line. When I run basic statistics it treats all lines in the ROI as one so I only get one line of statistics. I need all...

15 May 2018 3,736 2 View

What is the best strategy to introduce a HA-tag?

Dear All, I have a protein of interest that is fused to GFP at its C-terminus. I would like to add a HA tag at the N-terminus, and was wondering what strategy to use. Should I just introduce the...

08 September 2016 4,201 5 View

What are the most appropriate statistics to use for my experimental design?

11 May 2016 7,914 3 View

How do I stop RIPA-insoluble, Urea-soluble protein fraction from precipitating in Western blot wells?

18 November 2015 2,156 7 View

Which fluorophores are stable at pH 5?

Does anyone know which fluorophores are stable at low pH? I'm doing ISH combined with cell surface staining and then analysing the cells by flow cytometry. Sadly, the ISH needs to be carried out...

21 February 2012 6,730 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View