Hi there,
Is there any scientists who are doing some researches on exosomes and know a good way to store the isolated exosomes? Basically, we store the exosomes with PBS in -80°C, but we are afraid that the exosomes will be distroyed if stored in -80°C. Therefore, I am wondering if there are some good ways for the storage of exosomes, such as a specific buffer or something like that?
Many thanks!
I strongly recommend to follow a protocol proposed in this article:
Trehalose prevents aggregation of exosomes and cryodamage.Bosch S, de Beaurepaire L, Allard M, Mosser M, Heichette C, Chrétien D, Jegou D, Bach JM.Sci Rep. 2016 Nov 8;6:36162. doi: 10.1038/srep36162.
"Repeated freeze-thaw cycles induce an increase in particle concentration and in the width of the size distribution for exosome-like vesicles stored in PBS, but not in PBS 25 mM trehalose."
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