Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

How to thaw whole blood for ELISA?

Hi all, I have frozen (-20C) samples of whole blood from mice from a recent experiment. I need to find a way to thaw them so as to extract sera for an ELISA, while minimizing hemolysis and...

08 December 2020 1,004 3 View

Is PBS a suitable vehicle for testing the hemolytic activity of drugs in Ex vivo red blood cell hemolysis assay?

I am working with small molecules with antiplasmodial activity. I want to evaluate the hemolytic activity using a regular assay assessing hemolysis by measuring the absorbance at 414 nm.

19 November 2020 7,573 3 View

Does this unknown organism have alpha , beta or gamma haemolysis ?

I have ruined a part of the blood agar when I was streaking the plate which is making it difficult for me to analyse the results. I have included pictures for reference. Any help would be much...

11 December 2019 8,181 5 View

What type of Hemolysis is this?

I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.

03 April 2019 7,111 9 View

How do we calculate the %hemolysis of postive and negative control in rapid invitro hemolytic assay?

Recently I read many papers of reputed journals demonstrating the invitro cytotoxcity of synthetic materials, plant extracts, particles by Hemolytic assay, Triton-X100 is generally used as...

31 December 2018 4,725 7 View

Are assessment of hemolysis by absorbance at A414nm or calculations by M.Harboe method necessary?

Even mouse plasma that has (by observing visually) absorbance higher than 0.3 at A414nm. According to publications, this is a high level of hemolysis in humans. Does the same cutting point (0.3)...

06 July 2018 5,790 0 View

Is there a way to take into account the effect of hemolysis level to mouse plasma total RNA sequencing?

Several publications indicate hemolysis levels alter differential expression of a number of miRNAs in human plasma, in some cases up to 40-88% of miRNAs (does anyone have reference to such mouse...

06 July 2018 5,993 0 View

Is there any alternative reagent for (Triton X-100) to prepare a positive control used in the Hemolysis assay?

06 July 2018 9,209 2 View

Help with identifying probiotic class bacterial contamination?

-Plates exhibiting contamination are randomly throughout class. All class materials and equipment have been swabbed and checked as source with negative results (glass spreaders, turntables, agar,...

02 March 2018 5,353 9 View

Can someone help me come up with a good research title?

I can't decide of a concise title concerning my undergraduate thesis. Basically it deals with the bioactive secondary metabolites from Jellyfish-Associated marine fungi from the jellyfish...

02 March 2018 8,552 0 View