Shubin Fang

6 Questions 3 Answers 0 Followers

Questions related from Shubin Fang

Isolation of murine single lung cells?

Hi there, I have two questions for the isolation of murine single lung cells: 1. We use collagenase I for lung tissue digestion for 1h at 37 ℃ after cutting the lung tissues into small pieces....

26 September 2017 6,729 1 View

Storage of exosomes

Hi there, Is there any scientists who are doing some researches on exosomes and know a good way to store the isolated exosomes? Basically, we store the exosomes with PBS in -80°C, but we are...

29 June 2017 5,303 2 View

IL-33 induced ILC2-dominant asthma mouse model

Hi everyone, I am working on a project about the IL-33 induced ILC2-dominant asthma mouse model. However, I have a problem for building this animal model. I have read a lot of papers, and most...

29 June 2017 4,823 1 View

Airway smooth muscle cell

Hi, there! I am thinking about doing some projects on human airway smooth muscle cell. However, our lab is not affiliated to a hospital and it is very difficult to get the trachea tissue from the...

19 June 2017 1,148 0 View

Questions about the protocol for the LPS induced Th17-dependents neutrophilic asthma

Hi, everyone! I am doing project on the Th17-dependent neutrophilic asthma based on the papers published by Dr. Gregory S. Whitehead and Dr. Donald N. Cook as you can see in the attached...

19 July 2016 770 3 View

The definition of the "regulatory" macrophages

Hi, I am trying to working on the "regulatory" macrophages that were originally described in the paper titled Exploring the full spectrum of macrophage activation. However, I found that another...

01 January 1970 7,953 3 View