Standard concentration of Wnt3a in Pancreatic Cancer Organoid Culture?

11 August 2022 1 5K Report

I am receiving established orgnaoids from a different lab who use Wnt3a conditioned media in their organoid culture. I will be using recombinant Wnt3a (5036-WN-010/CF, R&D) and am wondering what concentration to use. I have seen 4ng/mL or 30-100ng/mL. Is there a standard for pancreatic cancer organoids?

Hossein Jahedi

We have been using some PDAC organoids purchased from ATCC and the protocol provided by them has been working well for us - 50% of complete organoid media consists of Wnt-3A conditioned media from CRL-2647 cells.

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