Hello, I deleted a message by accident. Is there anyway to retrieve it?

Deloris Wallace-Brown @Deloris-Wallace-Brown

31 January 2024 1 526 Report

Abhishek Paswan

No, you can't bring it back.

More Deloris Wallace-Brown's questions See All

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Where Can I Find Scholarly Sources on the origin of Prime Numbers?

Who discovered Prime Numbers? I need scholarly journal references on the discovery of Prime Numbers by the Ancient Greeks, Egyptians, Chinese, and Indians. Any scholarly journal references...

18 May 2024 5,736 0 View

Is it meaningful to take mean averages of scores that range from 1 - 3?

Hi, if someone has data that are scored: 1 for poor, 2 for adequate and 3 for good. Can you take a mean average if a score of less than 1 is not possible? I'm thinking if a mean score of...

23 April 2024 6,366 1 View

Please can you send full pdf version asap?

Van Dellen, A., Harris, A., Merryweather, J., Simsek, C., & Pendlebury, G. (2021). What the psychiatrist needs to know about the coroner's court in England and Wales. BJPsych...

27 March 2024 4,409 1 View

When do you think, if ever, will artificial general intelligence (AGI) be achieved?

There is currently much speculation on when AGI may be achieved if at all. Recent advancements in AI technology from ChatGPT’s SORA to NVIDIA’s robotics have fueled the belief that AGI is near-...

21 March 2024 7,867 10 View

Research on California indigenous land management?

I am trying to find the works of a researcher (I think an anthropologist) who wrote on indigenous land management in California. In particular, he argued that this management was not as spatially...

10 February 2024 6,241 3 View

What can be the reason of having negative OCR values and warning errors in background wells when running Seahorse?

I'm running XF T Cell persistence assay in a mini Seahorse. I used to not have problems until I start getting some negatives values in OCR measurements, and also errors with both background wells,...

12 January 2024 4,455 0 View

Does anyone have experience with culturing cyanobacteria and maintaining nutrient conditions over a long period of time?

We have a large-scale number of sterile cyanobacteria cultures grown in BG-11 media. As the cyanobacteria grow, they use up the nutrients in the media. I was wondering if anyone had a method in...

10 January 2024 575 2 View

How do you quantify cells in coronal mouse sections using ImageJ?

Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...

20 December 2023 7,869 0 View

Has anyone experimented with APTES vs TMSPMA to bond COC to PDMS?

I was wondering if anyone has done a direct comparison for using (3-Aminopropyl)triethoxysilane "APTES" vs 3-(Trimethoxysilyl)propyl methacrylate "TMSPMA" as a surface coating/Functionalization...

30 November 2023 9,204 0 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View

I accidentally removed 5 publications a few minutes ago. How can I add them again to my profile?

23 July 2024 9,145 2 View

Do I need an antigen retrieval for a multiplex RNAscope experiment for adherent cells?

Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...

22 July 2024 3,334 0 View

Information is Stored by Context?

While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...

17 July 2024 3,237 10 View

My question is about errors in Ansys mechanical APDL? Data file file.db does not exist for RESUME?

I did a welding simulation using ANSYS MECHANICAL APDL. The solution was done, but I encountered this error in the output file (Data file file.db does not exist for RESUME). On the other hand, I...

11 July 2024 4,480 0 View

How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View

Is it recommended to use DNA LoBind tubes for protein extraction - and how, if at all, does it affect retrieval of proteins?

Hi, in our group we are trying to optimize a protein extraction protocol, and I've noticed DNA LoBind tube and DNA LoBind tips are being used. I wanted to know if it is ok to use DNA LoBind for...

30 June 2024 7,751 2 View

By accident I removed two of my publications? Can you restore them?

23 June 2024 4,794 1 View