I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the prokaryotic DNA will be amplified and submitted for sequencing.
The sample volume is somewhere between 60ul and 100ul. What is the correct heat/run Time, Temperature, and Vacuum Pressure settings for this? I have the SPD131DDA-115 model. Furthermore is there any sample preparation needed before I place the samples in there. I extracted the samples using Qiagen RNA/DNA extraction protocol and eluted the DNA with water.
Does anyone have a protocol I could look at?
Thanks!
Hi Nicholas,
There is no standard protocol to do it..just put your samples into the speedvac with lid open and select mode for aqueous phase..you may check the DNA concentration using nanospec every few minutes until it reach the concentration that you like (more than 50 ng/uL is more than enough for a standard PCR reaction)..
Hello Nicholas,
SpeedVac for genomic DNA might not be such a good option. This high molecular weight DNA might be very difficult to re-dissolve after drying. In my opinion air-drying of the samples is the best option. It takes longer, of course, but you will have less trouble in getting your samples into solution.
Regards, Christian
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