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Questions related from Nicholas Samuel Brown
I am running a co-culture experiment with HeLa (mammalian cells) and various bacteria (P. disiens, P. bivia, L. crispatus). I need to measure the viability of the HeLa cells following a...
07 July 2017 6,614 1 View
I have an issue. I need to measure cell viability of my HeLa (mammalian) cells that are in co-culture with bacterial cells (Prevotella, Lactobacillus). If used common MTT assay methods with a 96...
07 July 2017 8,076 4 View
I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...
07 July 2017 7,901 6 View
I run a co-culture experiment with HeLa (mammalian) cells and bacteria (P. bivia, P. disiens, L. crispatus). Following a co-incubation period i want to measure the viability of the HeLa cells...
07 July 2017 8,829 3 View
I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...
07 July 2017 6,130 2 View
I am performing a bacterial co-culture with Hela cells and various bacterium of interest such as P. disiens and P. bivia. I am wondering if siRNA itself works well with bacterial cells?...
04 April 2017 1,746 4 View