Nicholas Samuel Brown

6 Questions 6 Answers 0 Followers

Questions related from Nicholas Samuel Brown

Calcein AM assay for HeLa cell viability (with invasion of bacteria)?

I am running a co-culture experiment with HeLa (mammalian cells) and various bacteria (P. disiens, P. bivia, L. crispatus). I need to measure the viability of the HeLa cells following a...

07 July 2017 6,599 1 View

Cell viability assay specific to mammalian cells? CANNOT pickup bacterial cells

I have an issue. I need to measure cell viability of my HeLa (mammalian) cells that are in co-culture with bacterial cells (Prevotella, Lactobacillus). If used common MTT assay methods with a 96...

07 July 2017 8,062 4 View

Fold change calculations following a qPCR NOT calculated by 2^-(ddCT)?

I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...

07 July 2017 7,886 6 View

Penicillin/streptomycin (PEST) cocktail effective against bacterial invaders of mammalian cells?

I run a co-culture experiment with HeLa (mammalian) cells and bacteria (P. bivia, P. disiens, L. crispatus). Following a co-incubation period i want to measure the viability of the HeLa cells...

07 July 2017 8,815 3 View

SpeedVac settings for genomic DNA?

I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...

07 July 2017 6,122 2 View

Lipofectamine, siRNA transfection on bacterial co-culture?

I am performing a bacterial co-culture with Hela cells and various bacterium of interest such as P. disiens and P. bivia. I am wondering if siRNA itself works well with bacterial cells?...

04 April 2017 1,737 4 View