I am performing a bacterial co-culture with Hela cells and various bacterium of interest such as P. disiens and P. bivia. I am wondering if siRNA itself works well with bacterial cells? Furthermore I am wondering if lipofectamine can be used on specific bacteria and Hela cells?
The siRNA is going to be used to knock down various genes of interest.
If you could please include a link to literature or sources in your answer that would be great.
-Thank you!
Avoid using lipofectamine in this sort of studies. siRNA is fine. The problem with lipofectamine or lipofactamine 2000 (as it is commonly known) is that ; no doubt it will help in transporting the DNA across the cell membrane, but then it may not get through the nuclear membrane especially if it is undergoing mitosis (you know very well that unless the nuclear material gets in side the cell translation is impossible).
@ Jai Ghosh sir, Is there any alternative available of lipofectamine for transfection?
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