which specific stain is used for chromaffin cell of adrenal gland?
Types of stains for chromaffin include:
Modified Giemsa
Schmorl's
Wiesel's
How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.
08 September 2018 7,678 1 View
Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?
06 July 2017 3,686 13 View
Anyone has the standardized protocol for prepration of oral dose for rat by powdered curcumin ?
04 May 2017 2,014 2 View
how can we prepare homogente from tissue for total cholestrol estimation?
02 March 2017 1,099 1 View
how to calculate the specific activity of MDA by wilbur method ?
09 October 2016 6,445 1 View
how to calculate the specific activity of GPx by rotruck method ?
09 October 2016 2,217 2 View
how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?
08 September 2016 3,458 1 View
what is the differnce between competitive RT- PCR and real time PCR?
07 August 2016 4,340 0 View
can anyone give the protocol of giemsa satining of tissue ?
07 August 2016 6,126 5 View
Anyone has method of stadtmen for cholestrol detrmintaion ?
07 August 2016 8,262 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View