Why we used Φ29 DNA polymerase in rolling circle amplification instead of the Taq polymerase used in the general PCR reaction. any specific function of this polymerase with respect to rolling circle amplification?
It has higher processivity and has higher fidelity compared to Taq polymerase. It has also a strand displacement property that Taq pol hasn't and which is required for RCA.
It has higher processivity and has higher fidelity compared to Taq polymerase. It has also a strand displacement property that Taq pol hasn't and which is required for RCA.