Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

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Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,001 5 View

SOP for removing multilayer graphene from one side of the Ni foil and etching the Ni foil with FeCl3 solution?

I have CVD grown Graphene on two sides of the Ni foil. I have tried O2 plasma (Ar 15 sccm & O2 5 sccm) with 150 W power for about 5-10 mins (multiple trials) to remove graphene from one side...

27 December 2018 3,120 1 View

Is the Zero Shot Learning a good current research area or not?

I am going to do my research on Zero Shot Learning. Is it good area in recent trends...

03 December 2018 7,101 3 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

14 April 2018 6,421 2 View

Any simple protocol for whitefly DNA extraction?

Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...

09 March 2018 3,103 4 View

Insects for DNA extraction stored in ethanol post collection?

Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...

17 February 2018 452 2 View

Viral like RNA assemblies?

Sequencing of plant transcriptomes often gives rise to additional sequences called virus like RNA assemblies corresponding to genomic RNAs of viruses infecting these host plants. How the plants...

05 February 2018 9,009 1 View

What does the word canonical mean with respect to translation?

I came across a term like "non canonical translation in RNA viruses". What does the term canonical mean?...even though after a dictionary search I havent got a satisfied answer. Does the word...

27 January 2018 6,184 4 View

Sodium acetate storage?

Should 3M sodium acetetate prepared for molecular cloning be stored under refrigerated conditions? what is the reason if so?

06 January 2018 6,301 2 View

Dilution of antiserum and conjugate in ELISA?

During an ELISA test for plant virus, if the quantity of antiserum and conjugate is less, is it possible to dilute the antiserum and conjugate with water (ultrapure/milliq)?

03 January 2018 8,665 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View