What is the role of soduim azide in the flow cytometry procedure and is there any other chemical to replace it?
Sodium azide acts as a preservative in most antibodies. During flow cytometry it assists in preventing capping, shedding, and internalization of the antibody-antigen complex after the antibodies bind to the receptors. If you are worried it may affect your assay, I have seen people not use it with success as well. I am not aware of any substitutes.
Good luck,
~Adam
Sodium azide acts as a preservative in most antibodies. During flow cytometry it assists in preventing capping, shedding, and internalization of the antibody-antigen complex after the antibodies bind to the receptors. If you are worried it may affect your assay, I have seen people not use it with success as well. I am not aware of any substitutes.
Good luck,
~Adam
Totally agreed with Adam comment, it provide stability to antibody as well
Sodium azide, only prevents bacterial n fungal growth in a high protein containing pbs solution. I regularly use azide free 2%bsa in pbs for sorting when I need live cells. Absence of azide, doesn’t affect the antibody staining in any ways. If at all anything, you should not keep your cells at room temp for long in azide solutions because it will kill your cell faster than them being in azide free pbs-bsa buffer.
- Badges
- Science method