One of our suppliers introduced me to Cultrex® BME PathClear and Geltrex® Matrix as the alternatives to matrigel. I was wondering as if anybody has experience of using these products?
Hi Nasibe,
maybe you're still interested in this topic.
Just recently I've successfully transferred my cells (just one line) from Marigel/mTeSR to GelTrex/Essential8 conditions. I just asked for samples (free)! The morphology is great, however I will modify some of the parameters in near future and do some more tests, to verify the healthiness of my hiPSCs.
When transferring cells from feeder-conditions to feeder-independent, Matrigel seems to be a necessary intermediate step, with GelTrex many cells died...
Another alternative would be Vitronectin...
When I've finally decided what's the best for my cells, I'll let you know!
Hi Nasibeh,
I noticed your question, however I think the title deserves a bit of a wider response. There are multiple Matrigel alternatives, not only the ones which you have listed.
There are more defined hydrogel systems which allow for a control over the hydrogel in which cells are embedded, where I would argue Cellendes (https://www.cellendes.com/) and HyStem (https://esibio.com/index.php/hystem-c-hydrogels/) are competent, well established systems. Alternatively, if you want to embed cells in a less defined, but more specific environment like one derived from specific tissues, Xylyx (https://xylyxbio.com/products/) offers a wide variety of different products to suit those needs.
I have to admit however, I am biased as our company also offers a Matrigel alternative, which is both defined and specific (https://www.denovomatrix.com/products/screenmatrix). I hope my answer helps you make the most informed decision for your cell culture!
Actually, Geltrex is not a matrigel alternative.
Geltrex is a brand of matrigel. I have found it to be more consistent than other matrigel options I tried in the past.
TissueLabs™ offers MatriXpec™ 3D, which is a tissue-specific (!!!) alternative to Matrigel, particularly for 3D cell culture. Check it out at www.tissuelabs.com.
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